Recombinant plasmid DNA (pDNA) is produced in bacterial cultures, mostly in Escherichia coli (E. coli) in different scales from tube culture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors, including the vector type, the host strain, the insert, and the methodology chosen for cultivation and downstream purification.
In this webinar, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vector and strain selection, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters.
Scientists and technical personnel in academic and industrial setting, as well as students who start or currently work on plasmid production and want to improve their plasmid yields in tube, shake or fermenter cultures.
Key learning objectives:
- Learn about the different application fields of plasmid production
- Get an overview on the basic steps involved in plasmid production
- Learn what to consider in plasmid vector and host strain selection
- Learn how to optimize your plasmid culture in tube and shake flask culture
- Learn about stirred-tank fermenter culture set-up and scale-up considerations
- Get advice on harvesting and purification
- Live: “Ask the Expert” session – Deepen your knowledge and optimize your plasmid culture
Ines Hartmann – Application Specialist, Cell Handling, Eppendorf SE
Ines Hartmann is a application specialist cell handling and responsible for the shakers and mixers at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.
Stefan Schlößser – Global Marketing Manager, Bioprocess Center, Eppendorf SE
Dr. Stefan Schlößer is a scientific communications manager at the Eppendorf Bioprocess Center in Juelich, Germany. He joined the company in 2022 and is responsible for conveying scientific knowledge with a focus on the complex topic of bioprocessing. Before joining the company, he studied biology and successfully defended his PhD thesis at the RWTH Aachen University, Germany. During his academic career he gained experience in the fields of microbiology, virology and cell biology.