BEGIN:VCALENDAR VERSION:2.0 PRODID:-//swoogo.com//NONSGML kigkonsult.se iCalcreator 2.41.90// CALSCALE:GREGORIAN UID:33643439-3930-4461-b837-633939303630 BEGIN:VEVENT UID:f1bef5b998013bab5ebbba2b3c54967bb052bc94@swoogo.com DTSTAMP:20260815T091509Z DESCRIPTION:Recombinant plasmid DNA (pDNA) is produced in bacterial culture s\, mostly in Escherichia coli (E. coli) in different scales from tube cul ture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors\, including the vector type\, the h ost strain\, the insert\, and the methodology chosen for cultivation and d ownstream purification.\n\nIn this webinar\, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vec tor and strain selection\, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters. \n\nTarget groups:\nScientists and technical personnel in academic and ind ustrial setting\, as well as students who start or currently work on plasm id production and want to improve their plasmid yields in tube\, shake or fermenter cultures.\n\nKEY LEARNING OBJECTIVES:\n\n * Learn about the diffe rent application fields of plasmid production\n * Get an overview on the b asic steps involved in plasmid production\n * Learn what to consider in pl asmid vector and host strain selection\n * Learn how to optimize your plas mid culture in tube and shake flask culture\n * Learn about stirred-tank f ermenter culture set-up and scale-up considerations\n * Get advice on harv esting and purification\n * Live: “Ask the Expert” session – Deepen your k nowledge and optimize your plasmid culture\n\nSPEAKER: \n\nINES HARTMANN – APPLICATION SPECIALIST\, CELL HANDLING\, EPPENDORF SE\n\nInes Hartmann is a application specialist cell handling and responsible for the shakers an d mixers at the Eppendorf headquarter in Hamburg\, Germany. She joined the company in 2008 and has worked on several topics in cell handling\, liqui d handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin\, Germany and gained expertise in variou s laboratory techniques.\n\n \n\nSTEFAN SCHLÖSSSER – GLOBAL MARKETING MANA GER\, BIOPROCESS CENTER\, EPPENDORF SE\n\nDr. Stefan Schlößer is a scienti fic communications manager at the Eppendorf Bioprocess Center in Juelich\, Germany. He joined the company in 2022 and is responsible for conveying s cientific knowledge with a focus on the complex topic of bioprocessing. Be fore joining the company\, he studied biology and successfully defended hi s PhD thesis at the RWTH Aachen University\, Germany. During his academic career he gained experience in the fields of microbiology\, virology and c ell biology.\n\n  DTSTART:20220928T140000Z DTEND:20220928T153000Z LAST-MODIFIED:20260815T091509Z LOCATION:https://event.eppendorf.com/labchannel/v/s-965112 SEQUENCE:0 STATUS:CONFIRMED SUMMARY:Optimize Your Plasmid Yields - Key Success Factors in Production fr om Shaker Flask to Fermenter TRANSP:OPAQUE X-ALT-DESC;FMTTYPE=text/html:

Recombinant plasmid DNA (pDNA) is produced in bacterial cultures\, mostly in Escherichia coli (E. coli) in different scales from tube culture to fermenter. Successful pDNA production with con sistent high yields and quality relies on multiple factors\, including the vector type\, the host strain\, the insert\, and the methodology chosen f or cultivation and downstream purification.

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In this webinar\, we review the distinct steps in the plasmid production workflow and give tip s on optimization: from vector and strain selection\, to cultivation from shake flask to fermenter up to downstream processing like harvesting and p urification. The focus will be on the optimization of the cultivation in s hake flasks and fermenters.

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Target groups:
\nScientists and technical personnel in academic and industrial setting\, as well as stude nts who start or currently work on plasmid production and want to improve their plasmid yields in tube\, shake or fermenter cultures.

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Key learning objectives:

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Speaker:

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Ine s Hartmann – Application Specialist\, Cell Handling\, Eppendorf SE

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Ines Hartmann is a application specialist cell handling and re sponsible for the shakers and mixers at the Eppendorf headquarter in Hambu rg\, Germany. She joined the company in 2008 and has worked on several top ics in cell handling\, liquid handling and consumables. She obtained her d iploma degree in biology at the Humboldt University of Berlin\, Germany an d gained expertise in various laboratory techniques.

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Stefan Schlößser – Global Marketing Manager\, Bioprocess Center \, Eppendorf SE

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Dr. Stefan Schlößer is a scientific com munications manager at the Eppendorf Bioprocess Center in Juelich\, German y. He joined the company in 2022 and is responsible for conveying scientif ic knowledge with a focus on the complex topic of bioprocessing. Before jo ining the company\, he studied biology and successfully defended his PhD t hesis at the RWTH Aachen University\, Germany. During his academic career he gained experience in the fields of microbiology\, virology and cell bio logy.

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BEGIN:VALARM UID:39363030-3538-4336-a132-646163633639 ACTION:DISPLAY DESCRIPTION:Recombinant plasmid DNA (pDNA) is produced in bacterial culture s\, mostly in Escherichia coli (E. coli) in different scales from tube cul ture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors\, including the vector type\, the h ost strain\, the insert\, and the methodology chosen for cultivation and d ownstream purification.\n\nIn this webinar\, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vec tor and strain selection\, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters. \n\nTarget groups:\nScientists and technical personnel in academic and ind ustrial setting\, as well as students who start or currently work on plasm id production and want to improve their plasmid yields in tube\, shake or fermenter cultures.\n\nKEY LEARNING OBJECTIVES:\n\n * Learn about the diffe rent application fields of plasmid production\n * Get an overview on the b asic steps involved in plasmid production\n * Learn what to consider in pl asmid vector and host strain selection\n * Learn how to optimize your plas mid culture in tube and shake flask culture\n * Learn about stirred-tank f ermenter culture set-up and scale-up considerations\n * Get advice on harv esting and purification\n * Live: “Ask the Expert” session – Deepen your k nowledge and optimize your plasmid culture\n\nSPEAKER: \n\nINES HARTMANN – APPLICATION SPECIALIST\, CELL HANDLING\, EPPENDORF SE\n\nInes Hartmann is a application specialist cell handling and responsible for the shakers an d mixers at the Eppendorf headquarter in Hamburg\, Germany. She joined the company in 2008 and has worked on several topics in cell handling\, liqui d handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin\, Germany and gained expertise in variou s laboratory techniques.\n\n \n\nSTEFAN SCHLÖSSSER – GLOBAL MARKETING MANA GER\, BIOPROCESS CENTER\, EPPENDORF SE\n\nDr. Stefan Schlößer is a scienti fic communications manager at the Eppendorf Bioprocess Center in Juelich\, Germany. He joined the company in 2022 and is responsible for conveying s cientific knowledge with a focus on the complex topic of bioprocessing. Be fore joining the company\, he studied biology and successfully defended hi s PhD thesis at the RWTH Aachen University\, Germany. During his academic career he gained experience in the fields of microbiology\, virology and c ell biology.\n\n  TRIGGER:-PT15M END:VALARM END:VEVENT END:VCALENDAR